This form is for MHC Class II requests only


Click here for MHC Class I request form


The request form is used for review of the request.


** Note: One request form per tetramer

Important! Your request will not be considered unless all the fields on this form are filled.

(Click here to find out when the Tetramer Resource Committee meets again to review requests.)

Be sure to register with our reagent program if you are a new client.

(Click here to download the Registration Form)

 

Contact and Shipping Information


Investigator's Name:
Title:
Institution:
Department:
Street Address:
City, State, ZIP
Telephone Number:
Fax Number:
E-mail: (Please note: our system can only accept one email address here on this form.)
Shipping Company / Account No.
 

** All reagents will be shipped to the above address which cannot be a post office box. **

Currently Available Pre-Made MHC Class II Monomers and Tetramers

Designation Allele Source Peptide Fluorophore
I-A(b) gp 66-77 I-A(b) lymphocytic choriomeningitis virus glycoprotein 66-77 DIYKGVYQFKSV PE or APC
I-A(b) MOG 38 I-A(b) mouse myelin oligodendrocyte glycoprotein 38-49 GWYRSPFSRVVH PE or APC

I-A(b) Ova 323

 

 

 

I-A(b)

 

 

 

chicken ovalbumin 323-339**

 

**Please note: This Ova reagent is problematic. Very high concentrations of tetramer are required,and it still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.

ISQAVHAAHAEINEAGR

 

 

 

PE or APC

 

 

 
I-A(b) ESAT-6 I-A(b) M. tuberculosis 6kDa early secretory antigenic target 1-20 MTEQQWNFAGIEAAASAIQG PE or APC
I-A(b) Ag85B I-A(b) M. tuberculosis antigen 85-B precursor 280-294 FQDAYNAAGGHNAVF PE or APC
I-A(b) LTAg I-A(b) murine polyomavirus large T antigen 676-688 NEYLLPQTVWARF PE or APC
I-A(b) FMLV I-A(b) Friend murine leukemia virus envelope protein 123-141 EPLTSLTPRCNTAWNRLKL PE or APC
I-A(b) Ea I-A(b) mouse MHC class II antigen E alpha 50-66 ASFEAQGALANIAVDKA PE or APC
I-A(b) MHV I-A(b) murine hepatitis virus membrane protein M 133-147 TVYVRPIIEDYHTLT PE or APC
I-A(d) HA I-A(d) influenza A virus hemagluttinin 126-138 HNTNGVTAACSHE PE or APC
I-A(d) malE I-A(d) maltose binding protein malE 69-84 GYAQSGLLAEITPDKA PE or APC
I-A(d) Ova 323 I-A(d) chicken ovalbumin 323-339 ISQAVHAAHAEINEAGR PE or APC
I-A(d) PLL I-A(d) mouse H+/K+ ATPase 889-900 PLLCVGLRPQWE PE or APC
I-A(d) PIT I-A(d) mouse H+/K+ ATPase 630-641 PITAKAIAASVG PE or APC
I-A(g7) BDC 2.5 seq 1 I-A(g7) BDC 2.5 mimotope sequence 1 AHHPIWARMDA PE or APC
I-A(g7) BDC 2.5 seq 2 I-A(g7) BDC 2.5 mimotope sequence 2 RTRPLWVRME PE or APC
I-A(g7) BDC 15 I-A(g7) BDC 15 mimotope AAAAVRPLWVRMEAA PE or APC
I-A(g7) Ova I-A(g7) chicken ovalbumin 141-160 CARELINSWVESQTNGIIRN PE or APC
I-A(g7) CD23 I-A(g7) rat CD23 216-231 DLKGRLVSIHSQKEQD PE or APC
I-A(g7) E25B I-A(g7) rat/mouse E25B 112-126 YQTIEENIKIFEEDA PE or APC
I-A(g7) GAD65 I-A(g7) human glutamic acid decarboxylase 2 (65kDa) 524-543 SRLSKVAPVIKARMMEYGTT PE or APC
I-A(g7) PLP I-A(g7) mouse proteolipid protein 56-70 DYEYLINVIHAFQYV PE or APC
DRB1*0301 PLP DRB1*0301 human myelin proteolipid protein 97-108 RQIFGDYKTTIC PE or APC
DRB1*0301 hsp DRB1*0301 Mycobacterium heat shock protein 65 3-13 KTIAYDEEARR PE or APC
Patr DRB1*0701 NS4 Patr DRB1*0701 hepatitis C virus NS4 1790-1799 LMAFTAAVTS PE or APC
Patr DRB1*1001 NS3 Patr DRB1*1001 hepatitis C virus NS3 1248-1258 GYKVLVLNPSV PE or APC

 

One order contains 200 ug of class II protein.

Tetramer Requested



Funding and Project Information


FOR NEW AND REFILL REQUESTS: PLEASE COMPLETE ALL FIELDS BELOW (failure to complete all fields will delay review and approval process). (NIH funding is not a requisite for requesting tetramers)

Title of Current Research Grant(s) or Contract  supported by NIH for which tetramers will be used:

 
Grant or Contract Number (if any):

 
Please list the staff that will be using this reagent (include collaborators):

 

Positive Control to test the requested tetramer(s): Yes No

Please describe the positive control that you will use to test the requested tetramer. If you do not have a positive control, please describe the method you will use to determine that the tetramer is working in your laboratory.

 
Research Abstract: This abstract should include a brief description of the research project, experimental system, how the tetramer reagent will be used to facilitate the research project, and a justification as to why tetramer results are needed instead of or in addition to results obtained from assays such as cytokine ELISPOTS or cytokine capture assays that do not require precise identification of MHC restriction. (limit: 1000 words)

If this is a refill request, please also answer the following questions.




 

Have you submitted a feedback form on your original order?
**If you have not provided feedback to the NIH Tetramer Facility Manager, approval of your order could be delayed.**
Please go to our online feedback form and submit a complete report.

 

Please provide your reason for requesting additional tetramer.
(If the tetramer no longer works, please provide a brief
description of how the tetramer was stored and explain
the experimental conditions in which the tetramer failed to stain,
including the positive and negative controls that were used in the study.)

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Last updated 08/18/08