Custom synthesis of class II MHC tetramers

We are pleased to announce the availability of custom-made class II MHC tetramers. These molecules are produced in mammalian cells.

In some cases, the MHC allele is expressed with a human CLIP peptide tethered to it via a cleavable linker sequence. To synthesize tetramers, the hCLIP peptide is cleaved off and exchanged with the client's peptide of interest. The client must provide 1 mg of the peptide of interest, and the production of these reagents takes about two weeks.

Currently, there are five human alleles for which we can offer this type of service.

DRB1*0101 / DRA1*0101

DRB5*0101 / DRA1*0101

DRB1*1501 / DRA1*0101

DRB1*0401 / DRA1*0101

DRB1*0301 / DRA1*0101

In other cases, we synthesize the MHC allele with the client's epitope peptide tethered to the beta chain instead of the hCLIP peptide. The client is not required to provide a peptide sample. However, the production of these tetramer reagents can take several months, since a new cell line expressing the MHC allele with the tethered peptide must be generated for each unique request.

Currently, there are eight mouse alleles and two chimpanzee alleles for which we can offer this type of service.

I-A(b)

I-A(d)

I-A(g7)

I-A(s)

I-A(u)

I-E(d)

I-E(k)

I-E(u)

Patr-DRB1*0701

Patr-DRB1*1001

In addition to the custom class II tetramers described above, the following  pre-made tetramers are also currently available. Again, the client is not required to provide a peptide sample when requesting these reagents. These also are produced as tethered constructs.

I-A(b) with lymphocytic choriomeningitis virus glycoprotein 66-77

I-A(b) with chicken ovalbumin 323-339**

**Please note: This Ova reagent is problematic. Very high concentrations of tetramer are required,and it still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.

I-A(b) with mouse myelin oligodendrocyte glycoprotein (MOG) 38-49 

I-A(b) with Mycobacterium tuberculosis 6kDa early secretory antigenic target (ESAT-6) 1-20 

I-A(b) with Mycobacterium tuberculosis antigen 85-B precursor 280-294 

I-A(b) with murine polyomavirus large T antigen 676-688 

I-A(b) with Friend murine leukemia virus envelope protein 123-141 

I-A(b) with mouse MHC class II antigen E alpha 50-66 

I-A(b) with murine hepatitis virus membrane protein M 133-147 

I-A(d) with maltose binding protein malE 69-84 

I-A(d) with influenza A virus hemagglutinin 126-138

I-A(d) with chicken ovalbumin 323-339

I-A(d) with mouse H+/K+ ATPase 889-900

I-A(d) with mouse H+/K+ ATPase 6330-641

I-A(g7) with BDC 2.5 mimotope sequence 1

I-A(g7) with BDC 2.5 mimotope sequence 2

I-A(g7) with BDC 15 mimotope 

I-A(g7) with chicken ovalbumin 142-160 

I-A(g7) with rat CD23 216-231 

I-A(g7) with rat/mouse E25B 112-126 

I-A(g7) with human/rat/mouse GAD65 524-543 

I-A(g7) with mouse PLP 56-70 

DRB1*0301 with human myelin proteolipid protein 97-108 

DRB1*0301 with Mycobacterium heat shock protein 65 3-13 

Patr DRB1*0701 with hepatitis C virus NS4 1790-1799 

Patr DRB1*1001 with hepatitis C virus NS3 1248-1258 

Our facility will be able to provide additional alleles in the future. DRB1*0701 and DRB1*0901 are the next alleles in the pipeline.  If there are other alleles your lab would like to see included in our class II production pipeline, please contact Amy Stout .